Seeing the Dynamics of Proteins and Nanoparticles in a New Light acsnano spectroscopy
By Bhavna KavetiSep 5 2022Reviewed by Susha Cheriyedath, M.Sc. . However, such measurements cannot be determined when the samples do not scatter enough light due to low concentrations of scatterers or small scattering cross sections.
This technique was applied in miniaturized microfluidics for light scattering and to access short-time dynamics of samples with low turbidity. The sensitivity gain in the present light scattering method was determined by measuring the diffusion coefficient and particle size. Protein dynamics is a highly complex phenomenon comprising numerous contributions from motions with different mechanisms of action and happening with diverse timescales and amplitudes that highly depend on the system and the local environment.
Moreover, due to the static nature of the scattered light, the resulting 3D interference pattern fluctuated for only dynamic samples. Hence, the CASS setup was efficient in studying the short-time dynamics of dilute samples, whose concentration was lower than the samples in traditional DLS measurement conditions.
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