'Jumping genes' help plants adapt to extreme temperature and pathogens oistedu NatureComms
Fourteen-day-old Col-0 and mutants were grown in a pot and 50 µM of ABA + 0.01% SILWET L-77 was sprayed onto the plants. Only water and 0.01% SILWET L-77 was sprayed onto the leaves of the mock plants. Biological replicates were harvested after 2 h of treatment and frozen in liquid nitrogen.The plants were subjected to heat shock stress as described by Ito et al., 2011. Twelve-day-old Col-0 were grown in pots at 21 °C before being cooled at 6 °C for 24 h.
For each genotype, around 80 plants were transferred into a glass plate containing 80 mL buffer , as previously described. The plants were incubated for 30 min in the buffer and covered with a transparent tissue layer that was engulfed by the solution. The plants were kept under light and were slowly shaken. Portions of the plants were collected after 30 min incubation; this corresponded to the time 0.
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