The life below our feet: Team discovers microbes thriving in groundwater and producing oxygen in the dark mblscience NatureComms
= 3) and Loon River formations. One well was not assigned with a geological formation. For further details concerning the location and geology refer to the SI.All wells were sampled at least once, while select wells were sampled during different seasons , or repeatedly to obtain sampling replicates . Some wells were installed as clusters, with multiple wells at the same location completed at different depths within the same formation or across multiple formations.
. The health of the sensor cap was tracked by monitoring optical dissolved oxygen gain within 0.7 to 1.4 and replaced every ~2 years.Groundwater samples for major cations and anions were collected in polyethylene bottles and analyzed at ALS Global by Inductively Coupled Plasma-Mass Spectrometry and Ion Chromatography, respectively. Total and phenolphthalein alkalinity was determined by titration, and bicarbonate and carbonate concentrations were subsequently calculated.
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