In recent years, the scientific community has made significant strides in the field of gene editing, particularly through the development of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) systems.
Nanjing Agricultural University The Academy of ScienceSep 25 2024 In recent years, the scientific community has made significant strides in the field of gene editing, particularly through the development of the CRISPR systems. In 2020, the Nobel Prize in Chemistry was awarded to the scientists for the discovery of CRISPR – Cas9 system, a revolutionary genome editing technology that advanced DNA therapeutics.
RNA plays a central role in carrying genetic information from DNA to protein-synthesizing machinery, and also regulates gene expression and participates in numerous cellular processes. Defects in RNA splicing or mutations can lead to a wide variety of diseases, ranging from metabolic disorders to cancer. A point mutation occurs when a single nucleotide is erroneously inserted, deleted, or changed.
The success of RNA-targeting CRISPR tools depends on multiple factors, including the guide RNA sequence, the accessibility of the target RNA, and the secondary structure of the RNA molecule. The CRISPR–Cas13 system has wide clinical applications, such as diagnostics for RNA viruses, RNA imaging, RNA-base editing, RNA epigenome editing, and therapeutic interventions.
CRISPR RNA Cas9 DNA Gene Gene Expression Genetic Genome Genome Editing Molecular Biology Mutation Palindromic Repeats Protein Research Splicing Technology Therapeutics Virus
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